Selenium is a carcinogenic metal and its deposits are found in ores containing other metals and elements. Percolation of selenium from its natural ores in rocks may prove to be hazardous to our delicate eco system. They will be a threat to human and wild life and they may also affect the vegetation in wetlands. Therefore selenium analysis in the laboratories is made mandatory for mining operations. It is considered as a priority pollutant in North America and mining operations there are subject to stringent regulations for selenium discharges. Compounds of selenium are widely used in rubber, metal alloys, medical therapeutic agents, glass, and pigments and in electronic and photocopier components.

Behavior of individual selenium species differ in all industrial and environmental processes, therefore selenium speciation has become necessary in order to analyze individual species and their importance as a nutrient that is vital for the existence of life. Selenium in mild concentration is a necessity whereas a concentrated dosage may have carcinogenic effect affecting health of both life and soil test. A new method which is comparatively faster and cheaper than many of the existing methods for direct speciation of organic and inorganic selenium species that is dissolved in biological and environmental samples was developed by dual column flow injection (FI) dual-column separation/ pre-concentration online along When the sample solution was passed through the column 1, separation of inorganic selenium and organic selenium could be achieved first with ICP-MS determination.

In this newly employed technique of selenium speciation, the first column which is packed with Al (2)O (3) of nanometer size absorb the inorganic species of selenium[Se(IV), Se(VI)], and inorganic selenium that remains is further eluted by0.2 mol l(-1) NaOH, whereas the organic selenium will not be retained. By passing sample solution through column 1 we can separate organic selenium and inorganic selenium. Things that affect the separation process will be investigated separately and conditions ideal for normal separation will be established.

Selenium being an essential nutrient and at the same time highly toxic when its concentration is more needs deeper study. Selenium analysis is undertaken carefully because of this dual effect and the studies has been extended to many other chemicals that occur naturally. It is a well recognized fact that traces of such harmful chemicals are found to be beneficial and concentrated settlements are harmful both for life and vegetation. In the case of selenium, so far, no “safe harbor limit” is set. This is mainly because of its nature of occur in organic and non organic forms and also in ionic and covalent forms. This quality makes selenium speciation a necessity.